NAD+ Reconstitution Calculator

Q: How do you reconstitute NAD+?

To reconstitute NAD+, add bacteriostatic water (BAC water) directly to the lyophilized (freeze-dried) NAD+ powder in the vial. For a 500 mg vial, adding 10 ml of BAC water gives a concentration of 50 mg/ml (50,000 mcg/ml). Inject the BAC water slowly against the side of the vial and gently swirl — do not shake. Store reconstituted NAD+ in the refrigerator and use within 30 days.

Q: How much BAC water for 500mg NAD+?

The most common reconstitution for a 500 mg NAD+ vial is 10 ml of bacteriostatic water, which yields a concentration of 50 mg/ml. This makes dosing easy: 1 ml delivers 50 mg, 2 ml delivers 100 mg, and so on. Some protocols use 5 ml for a 100 mg/ml concentration, which reduces injection volume but requires more precise measurement.

Q: What is the typical NAD+ dose?

Typical NAD+ doses range from 100 mg to 500 mg per session, depending on the administration route and individual tolerance. Subcutaneous injections are often given at 100–200 mg per day. Intravenous (IV) infusions typically range from 250 mg to 1,000 mg, administered slowly over several hours. Always start at the lower end of the dosing range and increase gradually. NAD+ is measured in milligrams (mg) — note that 500 mg equals 500,000 mcg.

Q: How long does reconstituted NAD+ last?

Reconstituted NAD+ should be stored in the refrigerator (2–8°C) and used within 30 days when reconstituted with bacteriostatic water (BAC water). BAC water contains 0.9% benzyl alcohol which acts as a preservative and extends shelf life compared to sterile water. Keep the vial away from light and do not freeze. Discard if the solution appears cloudy, discolored, or contains particles.

Q: Can you take NAD+ subcutaneously?

Yes, NAD+ can be administered subcutaneously (SubQ injection under the skin). SubQ administration is more convenient than IV infusion and avoids the need for an IV setup. Subcutaneous NAD+ is typically dosed at 100–200 mg per injection. Some people experience a brief burning sensation at the injection site, which is normal. Rotate injection sites (abdomen, thigh, or upper arm) to minimize irritation. IV infusion delivers NAD+ more rapidly and is preferred for higher doses.

NAD+ is dosed in milligrams (mg), not micrograms (mcg)

The calculator uses mcg internally. For NAD+, enter your dose in the "Desired Dose (mcg)" field using the mcg equivalent:

NAD+ Dose (mg)	Enter in calculator (mcg)	= grams
50 mg	50,000 mcg	0.05 g
100 mg	100,000 mcg	0.1 g
200 mg	200,000 mcg	0.2 g
500 mg	500,000 mcg	0.5 g
1,000 mg	1,000,000 mcg	1 g

500 mg NAD+ = 500,000 mcg | 1 mg = 1,000 mcg

Vial Size (mg)

BAC Water Added (ml)

Desired Dose (mcg)

Micrograms (mcg)

Milligrams (mg)

Quick Reference

mcg	mg	Common Use
100 mcg	0.1 mg	Ipamorelin, Hexarelin, GHRP-6
200 mcg	0.2 mg	Semax, Sermorelin, Ipamorelin
250 mcg	0.25 mg	BPC-157, Selank
500 mcg	0.5 mg	Semaglutide starting dose, PT-141
1,000 mcg	1 mg	AOD-9604, GHK-Cu, PT-141
2,000 mcg	2 mg	TB-500, Tesamorelin
2,400 mcg	2.4 mg	Cagrilintide
5,000 mcg	5 mg	BPC-157 vial, Epithalon, MOTS-C
10,000 mcg	10 mg	Tirzepatide, PT-141 vials

How to read these results

With 500 mg NAD+ reconstituted in 10 ml BAC water, the concentration is 50 mg/ml (50,000 mcg/ml). A 500 mg dose requires drawing 10 ml — the full vial — which is best suited for IV infusion rather than a single SubQ injection.

For subcutaneous use, a 100 mg dose (100,000 mcg) draws 2 ml, and a 50 mg dose (50,000 mcg) draws 1 ml. Adjust the dose field above to see the exact volume and syringe units for your protocol.

NAD+ Dose Reference — 500 mg Vial + 10 ml BAC Water

At 50 mg/ml concentration (500 mg vial + 10 ml BAC water). Syringe units shown for a standard 100-unit insulin syringe.

Dose (mg)	Dose (mcg)	Volume (ml)	Syringe Units	Use Case
50 mg	50,000 mcg	1.00 ml	100 units	SubQ — low starting dose
100 mg	100,000 mcg	2.00 ml	200 units	SubQ — standard daily dose
150 mg	150,000 mcg	3.00 ml	300 units	SubQ — higher daily dose
200 mg	200,000 mcg	4.00 ml	400 units	SubQ — max practical SubQ
250 mg	250,000 mcg	5.00 ml	500 units	IV infusion
500 mg	500,000 mcg	10.00 ml	1,000 units	IV infusion — full vial

Note: volumes above 2 ml are typically administered via IV drip, not SubQ injection. Consult a healthcare provider for your specific protocol.

NAD+ Reconstitution Guide

What is NAD+?

Nicotinamide adenine dinucleotide (NAD+) is a coenzyme found in every living cell, essential for energy metabolism, DNA repair, and cellular signaling. NAD+ levels decline with age, and supplementation via IV infusion or subcutaneous injection has gained interest in longevity, cognitive performance, and addiction recovery protocols.

Reconstitution step by step

Allow the NAD+ vial and BAC water to reach room temperature.
Wipe the tops of both vials with an alcohol swab and allow to dry.
Draw the desired volume of BAC water into a syringe (10 ml for a 500 mg vial).
Inject the BAC water slowly down the inside wall of the NAD+ vial — not directly onto the powder.
Gently swirl the vial until the powder fully dissolves. Do not shake vigorously.
The solution should be clear and colorless. Discard if cloudy or discolored.
Label the vial with the date and store in the refrigerator. Use within 30 days.

SubQ vs IV administration

NAD+ can be given subcutaneously (SubQ) or intravenously (IV). SubQ injections are more convenient but may cause mild burning or discomfort at the injection site due to NAD+'s acidity. IV infusion bypasses this and allows higher doses to be delivered efficiently. For IV use, NAD+ is typically diluted in normal saline or sterile water and administered slowly over 1–4 hours.

SubQ doses are typically 50–200 mg per injection. IV doses are typically 250–1,000 mg per session. Always start at the lower end and titrate upward based on tolerance.

Disclaimer: This calculator is a dosing reference tool. NAD+ administration should be supervised by a qualified healthcare provider. This information is not medical advice.

Frequently Asked Questions

How do you reconstitute NAD+?

To reconstitute NAD+, add bacteriostatic water (BAC water) slowly against the inside wall of the vial containing the lyophilized NAD+ powder. For a 500 mg vial, 10 ml of BAC water is standard, yielding a 50 mg/ml concentration. Gently swirl — never shake — until fully dissolved. The solution should be clear and colorless. Store refrigerated and use within 30 days. Use the calculator above to compute the exact injection volume for your dose.

How much BAC water for 500mg NAD+?

The most common protocol is to add 10 ml of BAC water to a 500 mg NAD+ vial. This creates a concentration of 50 mg/ml, making dose math straightforward: every 1 ml = 50 mg of NAD+. Some practitioners prefer 5 ml (100 mg/ml) for a more concentrated solution, which reduces injection volume. The calculator above is pre-filled with the 10 ml standard — adjust the BAC Water field if your protocol differs.

What is the typical NAD+ dose?

Typical NAD+ doses vary by administration route. For subcutaneous injections, doses range from 50 mg to 200 mg per day. For IV infusions, doses are typically 250 mg to 1,000 mg per session, administered 1–5 times per week depending on the protocol. Remember: NAD+ is dosed in milligrams (mg). 500 mg = 500,000 mcg = 0.5 g. Always begin with the lowest effective dose and work upward under medical supervision.

How long does reconstituted NAD+ last?

Reconstituted NAD+ lasts up to 30 days when stored in the refrigerator (2–8°C) and reconstituted with bacteriostatic water. BAC water contains benzyl alcohol, a preservative that inhibits microbial growth. If reconstituted with plain sterile water (no preservative), use within 24–48 hours. Always inspect the solution before each use — discard if cloudy, discolored, or if particles are visible. Do not freeze reconstituted NAD+.

Can you take NAD+ subcutaneously?

Yes. Subcutaneous (SubQ) NAD+ administration involves injecting into the fatty tissue just under the skin, typically in the abdomen, thigh, or upper arm. SubQ is more convenient than IV infusion and does not require clinical staff. Doses of 50–200 mg are practical for SubQ use — volumes larger than 2 ml per injection site are generally uncomfortable. Some users experience a burning sensation, which can be reduced by injecting slowly and allowing the solution to warm to room temperature first.