TB-500 Dosage Calculator
Calculate exact TB-500 injection volumes. Pre-filled for a 5mg vial with 2ml BAC water (2,500 mcg/ml). Typical 2mg dose = 80 units.
TB-500 (Thymosin Beta-4) is a synthetic fragment of Thymosin Beta-4, a 43-amino-acid peptide naturally present in human cells. In animal models, Thymosin Beta-4 has been reported to influence actin regulation, cell migration, angiogenesis and inflammation, and it is studied for tendon, ligament and muscle repair. Human evidence for the TB-500 fragment specifically is limited, and it is not FDA-approved.
For a 5mg TB-500 vial: add 2ml of bacteriostatic water → concentration is exactly 2500 mcg/ml. For the standard 2000mcg (2mg) dose, inject 0.80ml (80 units) on a 100-unit syringe.
Dose Reference Table
Pre-calculated for a 5mg vial + 2ml BAC water = 2,500 mcg/ml concentration. Using a 100-unit (1ml) insulin syringe.
| Dose | Inject Volume (ml) | Syringe Units |
|---|---|---|
| 500mcg (0.5mg) | 0.200 ml | 20 units |
| 1000mcg (1mg) | 0.400 ml | 40 units |
| 1500mcg (1.5mg) | 0.600 ml | 60 units |
| 2000mcg (2mg) | 0.800 ml | 80 units |
| 2500mcg (2.5mg) | 1.000 ml | 100 units |
| 4000mcg (4mg) | 1.600 ml | 160 units |
About TB-500
TB-500 is a synthetic version of Thymosin Beta-4 (Tβ4), a naturally occurring peptide found in high concentrations in blood platelets and other tissues throughout the body. It is one of the most studied healing peptides in research settings, with a broad range of potential regenerative applications.
TB-500 is thought to work primarily by promoting actin polymerization, which is essential for cell migration and tissue repair. Research has explored its role in healing muscle, tendon, ligament, and skin injuries, as well as potential cardiac and neurological protective effects.
TB-500 is typically supplied in 5mg vials. Using 2ml of bacteriostatic water gives a concentration of 2,500 mcg/ml. A typical loading dose of 2mg (2000mcg) requires 0.8ml — or 80 units on a 100-unit insulin syringe. Maintenance doses are often lower, around 1–1.5mg twice weekly.
TB-500 has a longer half-life than BPC-157 ("several days"), which is why it is typically dosed twice weekly rather than daily. It is often combined with BPC-157 in a blend for synergistic healing effects.
How this calculator works
Every result comes from the same three formulas used for any lyophilized peptide: concentration (mcg/ml) = vial mg × 1,000 ÷ BAC water ml; injection volume (ml) = desired dose mcg ÷ concentration; syringe units = injection volume ml × 100 on a U-100 syringe.
For the preset TB-500 setup on this page: a 5mg vial mixed with 2ml of bacteriostatic water gives a concentration of 2,500 mcg/ml. A 2,000mcg dose then needs 0.80ml, which is 80 units on a 100-unit insulin syringe.
The full methodology, with worked examples and the assumptions behind each formula, is on the methodology page.
Accuracy & limitations
The arithmetic itself is exact — the same inputs always produce the same output. Real-world accuracy depends on things outside the calculator: actual vial contents can vary slightly from the labeled amount within manufacturing tolerance, the BAC water volume should be measured carefully, and syringe readings are taken at the bottom edge of the plunger. Reconstituted peptides also degrade over time (the common guidance is to use within about 28 days refrigerated), which the math does not model. This tool does arithmetic only — it does not recommend doses, and it is not medical advice.
Last reviewed: September 2026 · Content, formulas and sources checked by Kanika