SS-31 Dosage Calculator
Calculate SS-31 (Elamipretide) injection volumes. Pre-filled for a 50mg vial with 5ml BAC water (10,000 mcg/ml).
SS-31 (Elamipretide) is a mitochondria-targeted tetrapeptide that binds cardiolipin on the inner mitochondrial membrane; in preclinical studies it supports electron transport chain function and reduces oxidative stress. It is being studied for heart failure, age-related mitochondrial decline, and skeletal muscle recovery, and remains investigational (not FDA-approved).
For a 50mg SS-31 vial: add 5ml of bacteriostatic water → concentration is exactly 10000 mcg/ml. A commonly referenced 3000mcg (3mg) research dose = 0.30ml (30 units) on a 100-unit syringe.
Dose Reference Table
Pre-calculated for a 50mg vial + 5ml BAC water = 10,000 mcg/ml concentration. Using a 100-unit (1ml) insulin syringe.
| Dose | Inject Volume (ml) | Syringe Units |
|---|---|---|
| 1000mcg (1mg) | 0.100 ml | 10 units |
| 2000mcg (2mg) | 0.200 ml | 20 units |
| 3000mcg (3mg) | 0.300 ml | 30 units |
| 5000mcg (5mg) | 0.500 ml | 50 units |
| 10000mcg (10mg) | 1.000 ml | 100 units |
About SS-31
SS-31 (Szeto-Schiller peptide 31), also known as Elamipretide or MTP-131, is a cell-permeable tetrapeptide that specifically targets cardiolipin — a phospholipid found exclusively in the inner mitochondrial membrane. By interacting with cardiolipin, SS-31 helps maintain cristae structure, supports electron transport chain function, and reduces reactive oxygen species (ROS) production.
SS-31 has been studied for its potential benefits in cardiac function, skeletal muscle performance, kidney protection, and neurological conditions. It has been investigated in clinical trials for heart failure with preserved ejection fraction (HFpEF), Barth syndrome, and other mitochondria-related conditions. It is not FDA-approved and remains an investigational drug.
SS-31 is supplied in larger vials (commonly 50mg) because research doses are in the milligram range. Using 5ml of bacteriostatic water gives a concentration of 10,000 mcg/ml (10 mg/ml). A 3mg dose requires 0.3ml — 30 units on a 100-unit insulin syringe.
SS-31 is administered via subcutaneous injection. Research protocols vary widely from daily to several times weekly depending on the study objective.
How this calculator works
Every result comes from the same three formulas used for any lyophilized peptide: concentration (mcg/ml) = vial mg × 1,000 ÷ BAC water ml; injection volume (ml) = desired dose mcg ÷ concentration; syringe units = injection volume ml × 100 on a U-100 syringe.
For the preset SS-31 setup on this page: a 50mg vial mixed with 5ml of bacteriostatic water gives a concentration of 10,000 mcg/ml. A 3,000mcg dose then needs 0.30ml, which is 30 units on a 100-unit insulin syringe.
The full methodology, with worked examples and the assumptions behind each formula, is on the methodology page.
Accuracy & limitations
The arithmetic itself is exact — the same inputs always produce the same output. Real-world accuracy depends on things outside the calculator: actual vial contents can vary slightly from the labeled amount within manufacturing tolerance, the BAC water volume should be measured carefully, and syringe readings are taken at the bottom edge of the plunger. Reconstituted peptides also degrade over time (the common guidance is to use within about 28 days refrigerated), which the math does not model. This tool does arithmetic only — it does not recommend doses, and it is not medical advice.
Last reviewed: September 2026 · Content, formulas and sources checked by Kanika